pbstween (Thermo Fisher)
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pbstween
Pbstween, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbstween+20/Tween+20/10__7554_slash_elife__104164__3-506-15-13
Average 99 stars, based on 1 article reviews
Pbstween, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbstween+20/Tween+20/10__7554_slash_elife__104164__3-506-15-13
Average 99 stars, based on 1 article reviews
pbstween - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Priming of pancreatic cancer cells with bispecific antibody armed activated T cells sensitizes tumors for enhanced chemoresponsiveness Article Snippet: Whole cell lysates were incubated in sodium dodecyl sulfate (SDS) sample buffer under reducing conditions at 100°C for 10 minutes and run on a 4–20% SDS–polyacrylamide gel (Bio-Rad Laboratories) followed by electro-transferring proteins onto polyvinylidene difluoride (PVDF) membranes. .. The PVDF membranes, blocked with 5% bovine serum albumin in Article Title: Activation of TGR5 restores AQP2 expression via the HIF pathway in renal ischemia-reperfusion injury. Article Snippet: Renal ischemia-reperfusion (I/R) injury is associated with markedly reduced protein expression of aquaporins (AQPs).. Membrane G protein-coupled bile acid receptor-1 (TGR5) has shown protective roles in some kidney diseases.. The purpose of the current study was to investigate whether activation of TGR5 prevented the decreased protein expression of AQPs in rodents with renal I/R injury and potential mechanisms. Article Title: HSPA6 is an ulcerative colitis susceptibility factor that is induced by cigarette smoke and protects intestinal epithelial cells by stabilizing anti-apoptotic Bcl-XL. Article Snippet: After blocking with 0.5% skim milk in PBS containing 0.1% Tween-20 for 1 h at room temperature (RT), membranes were probed with the mouse monoclonal α-HSPA6 antibody (#P17066, 1:1000; Enzo Life Sciences, Raamsdonksveer, The Netherlands), rabbit polyclonal α-Bcl-XL (#sc-634, 1:1000; Santa Cruz, Heidelberg, Germany) or mouse monoclonal α-GAPDH (#CB1001, 1:50.000; Calbiochem, CA, USA) in 0.5% BSA, 0.1% PBSTween-20 overnight at 4 °C. .. Membranes were incubated with horseradish peroxidase conjugated secondary antibodies in 0.1% Article Title: CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression. Article Snippet: The membranes were probed with primary antibodies toward caspase-1 (Abcam, Cambridge, UK, ab138483), cleaved caspase-1 (Abcam, ab207802), caspase-11 (Abcam,ab22684), cleaved caspase-11 (Abcam, ab180673), USP46 (Abcam, ab88795), Tubulin (Cell Signaling Technology, Danvers, MA, USA, D65A4), GAPDH (Abcam, ab8245) and β-actin (Cell Signaling Technology, 8H10D10), respectively. .. After washing with other:Article Title: Experimental and Human Evidence for Lipocalin‐2 (Neutrophil Gelatinase‐Associated Lipocalin [NGAL]) in the Development of Cardiac Hypertrophy and Heart Failure Article Snippet: Protein measurement of Lcn2 by Western Blots Protein was extracted from left ventricle of 13 week old HHR and NHR by the use of RIPA buffer (Sigma-Aldrich) and 1% Western Blot:Article Title: CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression. Article Snippet: The membranes were probed with primary antibodies toward caspase-1 (Abcam, Cambridge, UK, ab138483), cleaved caspase-1 (Abcam, ab207802), caspase-11 (Abcam,ab22684), cleaved caspase-11 (Abcam, ab180673), USP46 (Abcam, ab88795), Tubulin (Cell Signaling Technology, Danvers, MA, USA, D65A4), GAPDH (Abcam, ab8245) and β-actin (Cell Signaling Technology, 8H10D10), respectively. .. After washing with |